Urea, fluid

Equipment : Roche Cobas Pro c503

Method : Kinetic test with urease and glutamate dehydrogenase.

Urea is hydrolyzed by urease to form ammonium and carbonate. In the second reaction 2‑oxoglutarate reacts with ammonium in the presence of glutamate dehydrogenase (GLDH) and the coenzyme NADH to produce L‑glutamate. In this reaction two moles of NADH are oxidized to NAD+ for each mole of urea hydrolyzed.