Anti-Double Stranded DNA (Crithidia)

Method: Indirect immunofluorescence using Crithidia luciliae is used for the specific detection of IgG antibodies against double-stranded deoxyribonucleic acid (dsDNA). Crithidia luciliae flagellates are incubated with a diluted patient serum sample. If a positive reaction is obtained, specific antibodies of IgG, IgA and IgM class attach to the antigens. In a second step, the attached antibodies are stained with fluorescein-labelled anti-human IgG antibodies and made visible with a fluorescence microscope. Reference: Euroimmun IIFT: Crithidia luciliae (anti-dsDNA) Instructions for the indirect immunofluorescence test. Version: 10/01/2017.