Testosterone, serum

Equipment : Roche Cobas Pro e801

Method : Competition principle. Total duration of assay: 18 minutes.
▪ 1st incubation: 12 µL of sample are incubated with a biotinylated monoclonal testosterone‑specific antibody. The binding sites of the labeled antibody become occupied by the sample analyte (depending on its concentration).
▪ 2nd incubation: After addition of streptavidin-coated microparticles and a testosterone derivate labeled with a ruthenium complex, the complex becomes bound to the solid phase via interaction of biotin and streptavidin. The reaction mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the electrode. Unbound substances are then removed with ProCell II M. Application of a voltage to the electrode then induces chemiluminescent emission which is measured by a photomultiplier.
▪ Results are determined via a calibration curve which is instrument specifically generated by 2‑point calibration and a master curve provided via the cobas link.