NT-proBNP, blood

Equipment : Roche Cobas Pro e801

Method : Sandwich principle.
Total duration of assay: 18 minutes.
▪ 1st incubation: Antigen in the sample (9 µL), a biotinylated monoclonal NT‑proBNP‑specific antibody, and a monoclonal NT‑proBNP‑specific antibody labeled with a ruthenium complexa) form a sandwich complex.
▪ 2nd incubation: After addition of streptavidin-coated microparticles, the complex becomes bound to the solid phase via interaction of biotin and streptavidin.
Total duration of assay: 9 minutes.
▪ During a 9 minute incubation, antigen in the sample (9 µL), a biotinylated monoclonal NT‑proBNP‑specific antibody, a monoclonal NT‑proBNP‑specific antibody labeled with a ruthenium complex and streptavidin-coated microparticles react to form a sandwich complex, which is bound to the solid phase.
For both assay applications : The reaction mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the
electrode. Unbound substances are then removed with ProCell II M. Application of a voltage to the electrode then induces chemiluminescent emission which is measured by a photomultiplier.
▪ Results are determined via a calibration curve which is instrument specifically generated by 2‑point calibration and a master curve provided via the cobas link