BCR-ABL1, p190 & p210 breakpoints, RT-PCR Fusion Transcript Detection

Alternate test name

BCR-ABL1 Reverse Transcription Polymerase Chain Reaction (RT-PCR) Qualitative Testing.

Gene name / Alternate gene name

Philadelphia Chromosome, Ph, t(9;22), BCR-ABL Fusion, BCR-ABL1 Fusion, Chromosome 9 & 22 fusion, Chromosome 9 & 22 translocation, t(9;22)(q34;q11.2)

Lab area

Haematopathology - Molecular

Method and equipment

Reverse Transcriptase PCR followed by Gel Electrophoresis.

Expected turn-around time

3 Business Days

Specimen type

Bone Marrow Aspirate or Blood in EDTA (Lavender top tube) at room temperature (minimum 2mL)

Specimen requirements

Bone Marrow Aspirate or Blood in EDTA (Lavender top tube) at room temperature (minimum 2mL)

Storage and transportation

Keep specimen at room temperature

Shipping information

The Hospital for Sick Children

Rapid Response Laboratory

555 University Avenue, Room 3642

Toronto, ON

Canada

M5G 1X8

Phone: 416-813-7200

Toll Free: 1-855-381-3212

Hours: 7 days/week, 24 hours/day

Requisition

BCR-ABL1, p190 & p210 breakpoints, RT-PCR Fusion Transcript Detection - requisition

Background and clinical significance

The BCR-ABL1 fusion is an abnormal gene transcript formed by the translocation of the BCR (Breakpoint Cluster Region) gene on chromosome 22 and the ABL1 (ABL Proto-Oncogene 1, Non-Receptor Tyrosine Kinase) gene on chromosome 9. This fusion is most commonly associated with chronic myeloid leukemia (CML) and some cases of acute lymphoblastic leukemia (ALL). The fusion creates a new, abnormal protein that drives uncontrolled cell proliferation.