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Direct 16S PCR
Alternate test name
Gene name / Alternate gene name
16S rRNA
Lab area
Microbiology - General
Method and equipment
Polymerase Chain Reaction followed by Next Generation DNA sequencing
Expected turn-around time
14 days
Specimen type
Sterile body fluids including:
- CSF
- joint fluid
- pleural fluid
- pericardial fluid
- peritoneal fluid
Specimen requirements
- Minimum 0.5 mL of fluid specimen in sterile screw top tube/container
Storage and transportation
- All specimens must be shipped on dry ice
- Exception: Specimens that will arrive at SickKids within 48 hours from the time of collection can be shipped on ice packs
Special requirements
Testing dictated by demand.
Requisition
Disease condition
- Bacterial meningitis
- Septic arthritis
- Pleural effusion
- Pericarditis
- Peritonitis
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