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Follicle Stimulating Hormone (FSH), serum or plasma
Alternate test name
FSH, Gonadotropins include both FSH and LH
Lab area
Clinical Biochemistry - General
Method and equipment
Equipment : Roche Cobas Pro e801
Method : Sandwich principle. Total duration of assay: 18 minutes.
▪ 1st incubation: 24 µL of sample, a biotinylated monoclonal FSH‑specific antibody, and a monoclonal FSH‑specific antibody labeled with a ruthenium complexa) form a sandwich complex.
▪ 2nd incubation: After addition of streptavidin-coated microparticles, the complex becomes bound to the solid phase via interaction of biotin and streptavidin.
▪ The reaction mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the electrode. Unbound substances are then removed with ProCell II M. Application of a voltage to the electrode then induces chemiluminescent emission which is measured by a photomultiplier.
▪ Results are determined via a calibration curve which is instrument specifically generated by 2‑point calibration and a master curve provided via the cobas link.
▪ 1st incubation: 24 µL of sample, a biotinylated monoclonal FSH‑specific antibody, and a monoclonal FSH‑specific antibody labeled with a ruthenium complexa) form a sandwich complex.
▪ 2nd incubation: After addition of streptavidin-coated microparticles, the complex becomes bound to the solid phase via interaction of biotin and streptavidin.
▪ The reaction mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the electrode. Unbound substances are then removed with ProCell II M. Application of a voltage to the electrode then induces chemiluminescent emission which is measured by a photomultiplier.
▪ Results are determined via a calibration curve which is instrument specifically generated by 2‑point calibration and a master curve provided via the cobas link.
Expected turn-around time
STAT/ Urgent: 4 hours
Routine: 24 hours
Specimen type
Serum, Plasma Lithium Heparin
Specimen requirements
150 uL
Storage and transportation
Frozen
Shipping information
The Hospital for Sick Children
Rapid Response Laboratory
555 University Avenue, Room 3642
Toronto, ON
Canada
M5G 1X8
Phone: 416-813-7200
Toll Free: 1-855-381-3212
Hours: 7 days/week, 24 hours/day
Background and clinical significance
FSH and LH control growth and reproductive activities of the gonadal Tissues FSH promotes follicular development in the ovary and gametogenesis in the testis.
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Customer Service
Toll Free: 1-855-381-3212
Local: 416-813-7200