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Haemoglobin, plasma

Alternate test name

Haemolysis, Plasma Haemoglobin , Plasma Free Hemoglobin.

Lab area
Clinical Biochemistry - General
Method and equipment
Spectrophotometric
Expected turn-around time
STAT: 2 hours, Urgent: 4 hours., Routine: 24 hours
Specimen type

Sodium or lithium heparin plasma

Specimen requirements
  • Sample volume: 1200 uL (minimum 600uL)
  • Separation: Spin 20 minutes in centrifuge at 2000 rpm and separate into a12x75 mm plastic tube.
Storage and transportation

Frozen

Shipping information
The Hospital for Sick Children
Rapid Response Laboratory
555 University Avenue, Room 3642
Toronto, ON
Canada
M5G 1X8
Phone: 416-813-7200
Toll Free: 1-855-381-3212
Hours: 7 days/week, 24 hours/day
Background and clinical significance

Clinically, the presence of free hemoglobin after cardiac surgery suggests the occurrence of intravascular hemolysis which may require immediate action.

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