Fatty Acids, Free, serum

Alternate test name

Free Fatty Acid

Non-esterified Fatty Acid

Lab area

Clinical Biochemistry - General

Method and equipment

Equipment : Roche Cobas Pro c503

Method : NEFA is a colorimetric method.

The Randox enzymatic method relies upon acylation of coenzyme A (CoA) by the fatty acids in the presence of acyl-CoA synthetase (ACS). The acyl-CoA produced in the reaction is further oxidized by acyl-CoA oxidase (ACOD) with generation of hydrogen peroxide. Hydrogen peroxide, in the presence of peroxidase (POD) permits the oxidative condensation of N-ethyl-N-(2hydroxy-3-sulphopropyl)-m-toluidine (TOOS) with 4-aminoantipyrine (4-AAP) to form a purple coloured adduct which can be measured at 548 nm.

Expected turn-around time

STAT/ Urgent/ Routine: 7 days

Specimen type

Serum

Specimen requirements

150 uL

Storage and transportation

Specimen from outside of the hospital to be spun, separated, frozen and shipped on dry ice.

Shipping information

The Hospital for Sick Children

Rapid Response Laboratory

555 University Avenue, Room 3642