300 uL Serum or Plasma
- Collect 1mL whole blood, spin, separate and freeze (-20oC)
- Separated within 2 hrs of collection.
- Stored protected from light
- Unacceptable Specimens: Oxalate, citrate and EDTA cause significant losses of vitamin A and E
Stability: 1 month @ -20oC
Patient should be fasted for 12-14 h prior to sample collection.
No vitamin supplement within the previous 24 h.
No alcohol intake within the previous 24 h.
Indicate whether sample is from fasting patient or not
Vitamin A is critical for vision, growth, and many cell functions. High concentrations of vitamin A are seen with renal failure, but this is not associated with toxicity, and excessive ingestion. High concentrations are associated with bone fractures. Low concentrations of vitamin A are consistent with fat malabsorption and rarely due to inadequate diet. Vitamin A, vitamin E, and carotene are always extracted and detected simultaneously. This is a free vitamin A (retinol).
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