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Pyruvate, blood

Lab area
Clinical Biochemistry - Metabolic Diseases
Expected turn-around time
STAT/ Urgent/ Routine: 2 weeks
Specimen type

Blood or CSF Special Preparation

Specimen requirements

300 µL

Storage and transportation

Send frozen

Special requirements

Lactate must be done on same sample.

Pyruvate Sample Preparation Protocol
To be done within 30 minutes of blood being drawn.

Add 300 µL of heparinized whole blood to an eppendorf tube containing 300 µL of cold 6% Perchloric acid
Vortex for 30 seconds and keep on ice for 5 minutes
Spin down at 11,000 rpm
Transfer supernatant to a tube and freeze      

Shipping information
The Hospital for Sick Children
Rapid Response Laboratory
555 University Avenue, Room 3642
Toronto, ON
M5G 1X8
Phone: 416-813-7200
Toll Free: 1-855-381-3212
Hours: 7 days/week, 24 hours/day
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