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Bacterial 16S rDNA Detection from Clinical Specimens by Conventional Polymerase Chain Reaction (PCR)

Alternate test name

Gene name / Alternate gene name

16S rRNA

Lab area
Microbiology - Molecular
Method and equipment
Polymerase Chain Reaction followed by DNA sequencing
Specimen type

Sterile body fluids including:

  • joint fluid
  • pleural fluid
  • pericardial fluid
  • peritoneal fluid
Specimen requirements
  • Minimum 0.5 mL of fluid specimen in sterile screw top tube/container
Storage and transportation
  • All specimens must be shipped on dry ice
  • Exception: Specimens that will arrive at SickKids within 24 hours from the time of collection can be shipped on ice packs
Special requirements

Testing dictated by demand.

Disease condition
  • Septic arthritis
  • Pleural effusion
  • Pericarditis
  • Peritonitis
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