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Pancreatic Amylase, serum or plasma

Alternate test name

Pancreatic Isoamylase

Lab area
Clinical Biochemistry - General
Method and equipment
ABBOTT- Architect CI 4100
Expected turn-around time
STAT: 2 Hours Urgent: 4 Hours Routine: 24 Hours
Specimen type

Serum or Sodium/Lithium Heparin 

Specimen requirements

200 uL

Storage and transportation


Shipping information
The Hospital for Sick Children
Rapid Response Laboratory
555 University Avenue, Room 3642
Toronto, ON
M5G 1X8
Phone: 416-813-7200
Toll Free: 1-855-381-3212
Hours: 7 days/week, 24 hours/day
Background and clinical significance

The amylases are a group of hydrolases that split complex carbohydrates constituted of a-D-glucose units. The two recognized types of amylases are ß-amylase (e.g. plant and bacteria exoamylase) and human a-amylases which can attack the a-1,4-linkage anywhere along a polyglucan chain. Pancreatic amylase is synthesized only in pancreatic tissue by acinar cells, however the salivary glands and female genital, pulmonary tissues. Total amylase determinations used for the evaluation of pancreatic disorders often give misleading information due to high concentrations of salivary amylase present. The evaluation of pancreatic isoamylase would have a greater clinical specificity for the diagnosis of pancreatic disorders than total amylase assessment

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