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Neutrophil Oxidative Burst Index (NOBI)

Alternate test name
  • Nitro Blue Tetrazolium
  • NBT
Lab area
Method and equipment
  • BD FACSCanto
Expected turn-around time
Three Working Days
Specimen type
  • Peripheral Blood 
Specimen requirements
  • 2 mL EDTA
Storage and transportation
  • Keep blood at the room temperature.
  • If sample is from outside Toronto, keep at 2-8 degree (use an icepack).
  • Sample must be fresh (taken maximum 24 hours before testing).
  • Old sample can cause false results.
  • Include a transport control sample (an EDTA blood ~ 1 mL with normal CBC).
Special requirements
  • Flow Cytometry Laboratory only accepts samples Monday to Thursday from 8am to 4pm, Fridays 8am to 12pm excluding Holidays and Weekends.
  • Specimen stability in Flow testing is only acceptable within 24 hours of collection.
  • A complete Blood Count (CBC) and differential are required at the time of draw (Include a copy of the CBC and differential results).


Shipping information
The Hospital for Sick Children
Rapid Response Laboratory
555 University Avenue, Room 3642
Toronto, ON
M5G 1X8
Phone: 416-813-7200
Toll Free: 1-855-381-3212
Hours: 7 days/week, 24 hours/day
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