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Fluorescence in situ Hybridization (FISH) - Blood

Alternate test name

Rapid FISH (Direct Harvest)

  •  Trisomy 21
  •  Trisomy 13
  •  Trisomy 18
  •   X/Y
  •   BMT monitor by XX/XY FISH

FISH (Cultured Cells)

  •  Wolf-Hirschhorn (4p16)
  •  Williams (7q11.23)
  •  Prader-Willi/Angelman (15q11.2)
  •  Smith-Magenis (17p11.2)
  •  Microdeletion 22q11.2
  •  X/SRY
  •  SHOX (Xp22.3/Yp11.3)
  •  Microarray Follow Up FISH
  •  Other
Lab area
Genome Diagnostics - Cytogenetics
Method and equipment
3 day PHA-stimulated thymidine-synchronized culture; FISH (Fluorescence in situ hybridization)
Uncultured blood, direct harvest; Rapid FISH (Fluorescence in situ hybridization)
Expected turn-around time
Routine: 4 Weeks Newborn or expedite case: 5-9 working days Routine Microarray Follow Up FISH : 8-10 weeks
Specimen type

Peripheral blood

Specimen requirements

Blood at room temperature in sodium heparin
Volume: 0-3 months  1-3 mL
                3 months-12 years: 3-6 mL
                12 years - adult: 6 mL

Storage and transportation

Maintain at room temperature. Do not refrigerate or freeze.

Special requirements

Collect in sodium heparin tube

Shipping information
The Hospital for Sick Children
Division of Genome Diagnostics
555 University Avenue, Black Wing, Room 3416
Toronto, ON
M5G 1X8
Phone: 416-813-7200 ext. 2
Hours: Monday to Friday, 8 a.m. to 4:30 p.m.
Off hours: Please send to Rapid Response Laboratory, 555 University Avenue, Room 3642
Email Molecular Lab:
Email Cytogenetics:
Background and clinical significance


Disease condition


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