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Cortisol by LC-MS/MS

Alternate test name


Lab area
Clinical Biochemistry - Special
Method and equipment
Expected turn-around time
14 days
Specimen type

500 uL Serum or Plasma

Specimen requirements
  • Collect 1mL whole blood, spin, separate and freeze (-20oC)
  • Specimens collected on an SST tube should be separated within 90 minutes of receipt.
  • Avoid repeated freeze-thaw cycles.
Storage and transportation


Shipping information
The Hospital for Sick Children
Rapid Response Laboratory
555 University Avenue, Room 3642
Toronto, ON
M5G 1X8
Phone: 416-813-7200
Toll Free: 1-855-381-3212
Hours: 7 days/week, 24 hours/day
Background and clinical significance

Cortisol measurements are used as a direct monitor of adrenal status and an indirect measure of pituitary hyper or hypofunction. Elevated cortisol levels are associated with adrenal tumors, pituitary tumors or ectopic ACTH-producing tumors. Subnormal cortisol concentrations may indicate generalized adrenal hypofunction or a defect in the metabolic pathway for cortisol biosynthesis.

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