Lauryl M J Nutter

Dr. Nutter was trained as a geneticist using Drosophila as a model system but moved to using mice as a model system during her post-doctoral fellowship with Dr. Jayne Danska at The Hospital for Sick Children. Since 2007, Dr. Nutter has been designing and developing genetically engineered mouse lines to study development and disease across many biological domains, with more than 500 new mouse lines generated in her laboratory.

Research

The Nutter lab has been involved in producing new knockout mouse lines, using embryonic stem cells and Cas endonucleases, for the International Mouse Phenotyping Consortium since 2011. This international collaboration aims to produce and phenotype single-gene knockout mouse lines for all orthologous genes in the mammalian genome.

The Nutter lab is also interested in developing and optimizing tools used in genetic engineering, particularly for developing mouse lines and models relevant to studying human health and disease. The lab is currently investigating methodologies for humanizing genes, generating defined structural variants, as well as optimizing methodologies for more common alleles such as patient-associated variant and conditional alleles for lethal genes.

Education and experience

• 1997–2000: Post-doctoral Fellow, The Hospital for Sick Children, Toronto, Ontario, Canada.
• 1997–1997: Ph.D., Molecular Genetics, The University of Calgary, Calgary, Alberta, Canada.
• 1985–1990: B.Sc., Cellular, Molecular & Microbial Biology, The University of Calgary, Alberta, Canada.

Achievements

• 2019: International Mouse Phenotyping Consortium Award of Excellence.

1. Elrick H., Peterson K.A., Willis B.J., Lanza D.G., Acar E.F., Ryder E.J., Teboul L., Kasparek P., Birling M.C., Adams D.J., Bradley A., Braun R.E., Brown S.D., Caulder A., Codner G.F., DeMayo F.J., Dickinson M.E., Doe B., Duddy G., Gertsenstein M., Goodwin L.O., Herault Y., Lintott L.G., Lloyd K.C.K., Lorenzo I., Mackenzie M., Mallon A.M., McKerlie C., Parkinson H., Ramirez-Solis R., Seavitt J.R., Sedlacek R., Skarnes W.C., Smedley D., Wells S., White J.K., Wood J.A., International Mouse Phenotyping C., Murray S.A., Heaney J.D. & Nutter L.M.J. (2024) Impact of essential genes on the success of genome editing experiments generating 3313 new genetically engineered mouse lines. Sci Rep 14, 22626. PubMed PMID: 39349521.
2. Gertsenstein M., Lintott L.G. & Nutter L.M.J. (2025) Engineering Base Changes and Epitope-Tagged Alleles in Mice Using Cas9 RNA-Guided Nuclease. Curr Protoc 5, e70109. PubMed PMID: 39999224.
3. Gertsenstein M. & Nutter L.M.J. (2021) Production of knockout mouse lines with Cas9. Methods 191, 32-43. PubMed PMID: 33524495.
4. Lintott L.G. & Nutter L.M.J. (2023) Genetic and Molecular Quality Control of Genetically Engineered Mice . Methods Mol Biol 2631, 53-101. PubMed PMID: 36995664.
5. Peterson K.A., Khalouei S., Hanafi N., Wood J.A., Lanza D.G., Lintott L.G., Willis B.J., Seavitt J.R., Braun R.E., Dickinson M.E., White J.K., Lloyd K.C.K., Heaney J.D., Murray S.A., Ramani A. & Nutter L.M.J. (2023) Whole genome analysis for 163 gRNAs in Cas9-edited mice reveals minimal off-target activity . Commun Biol 6, 626. PubMed PMID: 37301944.

View a full list of Lauryl Nutter's publications: NCBI and Google Scholar.